application and adaptation of dot immunoassay to detect and measure rubella virus antibody

نویسندگان

t bamdad from the department of virology. facility a/medical sciences. tarbiat modarres university. po. box 14115-111. tehran

mh rodstai from the department of virology. facility a/medical sciences. tarbiat modarres university. po. box 14115-111. tehran

h solimandjahi from the department of virology. facility a/medical sciences. tarbiat modarres university. po. box 14115-111. tehran

m malekaneh the department a/biochemistry. faculty of medical sciences. birjand university of medical science's. birdjand. i.r. iran.

چکیده

a dot immunobinding assay ( dia) was used for a quantitative and qualitative assay of rubella antibody. purified antigen and conjugated anti-human immunoglobulin (rahlg) were prepared. nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. the reacting antibodies were visualized by a peroxidase system. development of a colored insoluble substrate was taken as a positive result. the adapted dia was applied to test 105 serum samples. the sensitivity and specificity and immune titer of dia were compared with hemagglutination inhibition (hi) test.

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APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY

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عنوان ژورنال:
medical journal of islamic republic of iran

جلد ۱۵، شماره ۳، صفحات ۱۷۱-۱۷۴

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